Histology fixative

Tissue fixation is used for several reasons, including prevention of putrefaction from bacteria, autolysis from enzyme degradation and loss of soluble substances. Most tissue fixation also helps enhance staining in later techniques like immunohistochemistry, if required and also routine haematoxylin and eosin staining.

Fixation preserves a sample of biological material (tissue or cells) as close to its natural state as possible in the process of preparing tissue for examination.

To achieve this, several conditions usually must be met.

Some of the more popular and traditional fixative solutions are shown below (click each line for details). Those chosen are simply representative of the major groups. Some of these reagents can be purchased from us ready-to-use.

 

Code Description Packaging
0207119 B5 fixative 6 x 500 ml
01BOU5000 Bouin Fixative 5 lt
01BOU1000 Bouin fixative 1 lt
0207116Z Bouin fixative concentrated solution 6 x 1 lt
07-118 Bouin Hollande fixative 20 x 500 ml
0207103 Buffered formalin OsM certified 20 lt
01BFC1000 Carnoy’s fixative 1000 ml
01BFC500 Carnoy’s fixative 500 ml
R0150 Cyto spray 12 x 250 ml
22-140 Cyto-d cytological fixative 1000 ml
22-141 Cyto-d red cytological fixative and small biopsies 1000 ml
01HLBFHD1000 Fixative Davidson Hartman 1000 ml
01F37H10000 Formaldehyde 37% for use in histology 10 lt
01F37H5000 Formaldehyde 37% for use in histology 5 lt
01F37H1000 Formaldehyde 37% for use in histology 1 lt
0207111 Formalin acetate buffer 4 x 5 lt
07-122 Formalin Acid Alcohol (F.A.A.) 1000 ml
07-112 Formalin fosfate buffer 4 x 5 lt
0207107 Formalin Millioning Carson pH 7,2-7,4 4 x 5 lt
00376-500 Glutaraldehyde, Biological Grade, 25% 4 x 500 ml
00376-4 Glutaraldehyde, Biological Grade, 25% 4 x 500 ml
00377-500 Glutaraldehyde, Biological Grade, 50% 500 ml
00377-4 Glutaraldehyde, Biological Grade, 50% 4 x 1000 ml
00377-1 Glutaraldehyde, Biological Grade, 50% 1000 ml
DR003 Histo-Fix - 10% neutral buffered formalin 20 lt.
DR002 Histo-Fix - 10% neutral buffered formalin 10 lt.
DR001 Histo-Fix - 10% neutral buffered formalin 4 x 5 lt.
16-100 Kaiserling I fixative 6x500 ml
16-101 Kaiserling II fixative 6x500 ml
MTF999 Michel's Transport Fluid 1 lt
MTF500 Michel's Transport Fluid 500 ml
MTF-10000 Michel's Transport Fluid 10 lt
07-115 NoTox - formalin substitute 4 x 5 lt
22-114 Saccomanno fixative 6 x 500 ml
24775-250 Soft Nail - Soften Keratin 250 ml
HL82076 Tap for container 5 pz.
07-120 Zamboni fixative 6 x 500 ml